Measurement of Nanomolar Dissociation Constants by

1 nanomolar [31]. Full-length yeast Hsp90 bound to radicicol with a Kd of 19 nM, and the N-terminal domain (1-220 a.a.) of yeast Hsp90 bound to radicicol with a Kd of 2.7 nM. As pointed out by the authors, the binding was too tight to be determined directly by ITC. Here, we determined the binding


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